Human RNF10 knockout HEK-293T cell lysate
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RNF10 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon1 and Insertion of the selection cassette in exon1.
View Alternative Names
AA675014, KIAA0262, MGC126758, MGC126764, RIE2, Ring finger protein 10, Sid 2705
- Sanger seq
Unknown
Sanger Sequencing - Human RNF10 knockout HEK-293T cell lysate (AB263329)
Allele-1 : 11 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human RNF10 knockout HEK-293T cell lysate (AB263329)
Allele-2 : Insertion of the selection cassette in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RNF10 participates in regulating cellular processes like neurodevelopment and muscle differentiation. It interacts with other proteins to form complexes that influence cell signaling pathways and transcription regulation. This protein's activities affect neuronal growth and synaptic plasticity important for learning and memory. RNF10 also has implications in muscle cells where it modulates the expression of genes related to muscle function.
Pathways
RNF10 takes part in the MAPK signaling pathway which plays a role in cell proliferation and differentiation. In this pathway RNF10 interacts with proteins such as MEK and ERK allowing it to influence downstream signaling processes. Additionally RNF10 has connections to the Wnt signaling pathway impacting gene expression patterns important for development. These interactions highlight its role in orchestrating complex cellular responses.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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