Human RNF167 knockout HEK-293T cell lysate
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RNF167 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
View Alternative Names
5730408C10Rik, E3 ubiquitin-protein ligase RNF167, LP2254, RING finger protein 167, RING105, RN167_HUMAN
- Sanger seq
Unknown
Sanger Sequencing - Human RNF167 knockout HEK-293T cell lysate (AB259087)
Homozygous : 1 bp insertion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The functions of RNF167 are pivotal in regulating the stability and turnover of membrane proteins which are key to cellular homeostasis. It plays a role in endosomal sorting and trafficking ensuring that proteins reach their proper intracellular destinations. RNF167 forms part of a larger complex involving the ESCRT machinery which is essential for multivesicular body pathway and lysosome degradation processes.
Pathways
RNF167 interacts in significant cellular mechanisms impacting protein degradation and recycling. It participates in the endosomal-lysosomal pathway closely interacting with other proteins like VPS29 which aids in the recycling of transmembrane receptors. The protein also has roles related to the ubiquitin-proteasome system intersecting with pathways involving protein quality control and it functions in concert with proteins like HRS in these processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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