Human RNF181 (LOC51255) knockout HEK-293T cell lysate
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RNF181 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.
View Alternative Names
1700047F16Rik, 2500002L14Rik, 6230415N01Rik, C77350, E3 ubiquitin protein ligase RNF181, HSPC238, MGC103058, MGC94464, OTTHUMP00000209531, OTTHUMP00000209532, RING finger protein 181, RNF181
- Sanger seq
Unknown
Sanger Sequencing - Human RNF181 (LOC51255) knockout HEK-293T cell lysate (AB258640)
Homozygous : 1 bp insertion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
LOC51255 regulates cellular processes including signal transduction and gene expression. It operates as part of a larger protein complex associated with ciliary functions. As a transcription factor LOC51255 influences the expression of genes related to cellular signaling pathways impacting various physiological responses and developmental processes.
Pathways
LOC51255 takes part in significant pathways such as the Hedgehog signaling and ciliogenesis. In the Hedgehog signaling pathway LOC51255 interacts with proteins like Gli1 to regulate the expression of downstream target genes. During ciliogenesis LZTFL1 coordinates with proteins like BBS7 and BBS1 highlighting its role in proper cilia formation and function.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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