Human RNF19B knockout A549 cell lysate
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RNF19B KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 11 bp deletion in exon2.
View Alternative Names
E3 ubiquitin-protein ligase RNF19B, FLJ90005, IBR domain containing 3, IBR domain-containing protein 3, IBRDC3, NKLAM, Natural killer lytic-associated molecule, RING finger protein 19B, RN19B_HUMAN, rnf19b
- Sanger seq
Unknown
Sanger Sequencing - Human RNF19B knockout A549 cell lysate (AB258642)
Homozygous : 11 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RNF19B participates in the endoplasmic reticulum-associated degradation (ERAD) pathway and interacts with different co-factors to form functional complexes. It facilitates the removal of misfolded or damaged proteins within the cell thereby maintaining protein homeostasis. The protein's ability to recognize and ubiquitinate misfolded proteins plays a critical part in cellular quality control mechanisms.
Pathways
RNF19B interacts significantly with the ubiquitin-proteasome system and also affects the Parkinson's disease pathway. RNF19B links to key proteins like parkin and CHIP which are involved in the degradation of proteins associated with neurodegenerative diseases. Through these interactions RNF19B modulates cell health and protein turnover highlighting its central role in maintaining cellular balance.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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