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RNF2 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 8 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.

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Images

Western blot - Human RNF2 (RING2 / RING1B) knockout HeLa cell lysate (AB257640), expandable thumbnail
  • Sanger Sequencing - Human RNF2 (RING2 / RING1B) knockout HeLa cell lysate (AB257640), expandable thumbnail
  • Sanger Sequencing - Human RNF2 (RING2 / RING1B) knockout HeLa cell lysate (AB257640), expandable thumbnail

Key facts

Cell type
HeLa
Species or organism
Human
Tissue
Cervix
Knockout validation
Sanger Sequencing
Mutation description
Knockout achieved by using CRISPR/Cas9, 8 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.

Alternative names

DING, DinG protein, E3 ubiquitin protein-ligase RING 2, E3 ubiquitin-protein ligase RING2, HIP2-interacting protein 3, HIPI 3, Huntingtin-interacting protein 2-interacting protein 3, OTTHUMP00000060668, Polycomb M33 interacting protein Ring 1B, Protein DinG, RING 1B, RING finger protein 1B, RING finger protein 2, RING finger protein BAP-1, RING2_HUMAN, RNF 2

What's included?

1 Kit
Components
Human RNF2 knockout HeLa cell lysate
1 x 100 µg
Human wild-type HeLa cell lysate
1 x 100 µg

Recommended products

RNF2 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 8 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.

Key facts

Cell type
HeLa
Mutation description
Knockout achieved by using CRISPR/Cas9, 8 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.
Disease
Adenocarcinoma
Concentration
Loading...

Properties

Gene name
RNF2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Notes

Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RING2 also known as RING1B or RNF2 is a protein that functions as a member of the Polycomb Repressive Complex 1 (PRC1). This protein contains a RING-finger domain facilitating ubiquitin ligase activity. It has a molecular mass of approximately 38 kDa. RING2/RNF2/RING1B is expressed in various tissues including the brain heart and lungs indicating its fundamental role across different biological systems.

Biological function summary

RING2/RNF2/RING1B regulates gene expression by modifying chromatin structure especially through monoubiquitination of histone H2A at lysine 119. This action occurs within the Polycomb Repressive Complex 1 a multiprotein structure important for maintaining the transcriptional repression of target genes. It influences cell cycle progression and development by silencing genes involved in proliferation and differentiation.

Pathways

RING2/RNF2/RING1B's role connects to the Polycomb group (PcG) pathway and the Wnt signaling pathway. Within these pathways it interacts with proteins like BMI1 and EZH2 which are other components of polycomb complexes. These interactions help modulate chromatin dynamics and control the expression of several genes involved in cell identity and development.

Associated diseases and disorders

Dysfunction or aberrant expression of RING2/RNF2/RING1B has links to cancer and neurological disorders. Its relationship with proteins like CBX8 ties it to pathways of oncogenesis where it can influence tumorigenesis through misregulation of gene silencing. Furthermore changes in RING2/RNF2/RING1B expression or function may contribute to developmental disorders associated with improper neuronal development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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