Human RNF2 (RING2 / RING1B) knockout HeLa cell lysate
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RNF2 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 8 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.
View Alternative Names
DING, DinG protein, E3 ubiquitin protein-ligase RING 2, E3 ubiquitin-protein ligase RING2, HIP2-interacting protein 3, HIPI 3, Huntingtin-interacting protein 2-interacting protein 3, OTTHUMP00000060668, Polycomb M33 interacting protein Ring 1B, Protein DinG, RING 1B, RING finger protein 1B, RING finger protein 2, RING finger protein BAP-1, RING2_HUMAN, RNF 2
- WB
Lab
Western blot - Human RNF2 (RING2 / RING1B) knockout HeLa cell lysate (AB257640)
Lane 1 : Wild-type HeLa cell lysate (20µg)
Lane 2 : RNF2 knockout HeLa cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab181140 observed at 42 kDa. Red - loading control, ab7291 observed at 50 kDa.
ab181140 Anti-RING2 / RING1B / RNF2 antibody [EPR12245] was shown to specifically react with RING2 / RING1B / RNF2 in wild-type HeLa cells in western blot. The band observed in the knockout cell line ab264845 (knockout cell lysate ab257640) lane below 42kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and RING2 / RING1B / RNF2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab181140 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-RING2 / RING1B / RNF2 antibody [EPR12245] (<a href='/en-us/products/primary-antibodies/ring2-ring1b-rnf2-antibody-epr12245-ab181140'>ab181140</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
Rnf2 (RING2 / RING1B) knockout HeLa cell lysate at 20 µg
Predicted band size: 37 kDa
Observed band size: 42 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human RNF2 (RING2 / RING1B) knockout HeLa cell lysate (AB257640)
Allele-1 : 8 bp deletion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human RNF2 (RING2 / RING1B) knockout HeLa cell lysate (AB257640)
Allele-2 : Insertion of the selection cassette in exon 2
Reactivity data
Product details
Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RING2/RNF2/RING1B regulates gene expression by modifying chromatin structure especially through monoubiquitination of histone H2A at lysine 119. This action occurs within the Polycomb Repressive Complex 1 a multiprotein structure important for maintaining the transcriptional repression of target genes. It influences cell cycle progression and development by silencing genes involved in proliferation and differentiation.
Pathways
RING2/RNF2/RING1B's role connects to the Polycomb group (PcG) pathway and the Wnt signaling pathway. Within these pathways it interacts with proteins like BMI1 and EZH2 which are other components of polycomb complexes. These interactions help modulate chromatin dynamics and control the expression of several genes involved in cell identity and development.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB181140
Anti-RING2 / RING1B / RNF2 antibody [EPR12245]
RabMAb|Recombinant|KO Validated
![Flow Cytometry (Intracellular) - Anti-RING2 / RING1B / RNF2 antibody [EPR12245] (AB181140)](https://content.abcam.com/products/images/ring2-ring1b-rnf2-antibody-epr12245-ab181140--flow-cytometry-intracellular-img50440.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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