RNF4 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 261 bp deletion in exon4 and Insertion of the selection cassette in exon4.
Images
Key facts
- Cell type
HeLa
- Species or organism
Human
- Tissue
Cervix
- Knockout validation
Sanger Sequencing
- Mutation description
Knockout achieved by using CRISPR/Cas9, 261 bp deletion in exon4 and Insertion of the selection cassette in exon4.
Alternative names
E3 ubiquitin ligase RNF4, E3 ubiquitin protein ligase RNF4, Protein SNURF, RES4 26, RING finger protein 4, RNF4_HUMAN, SLX5, Small nuclear ring finger protein
What's included?
Recommended products
RNF4 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 261 bp deletion in exon4 and Insertion of the selection cassette in exon4.
Key facts
- Cell type
HeLa
- Species or organism
Human
- Tissue
Cervix
- Knockout validation
Sanger Sequencing
- Mutation description
Knockout achieved by using CRISPR/Cas9, 261 bp deletion in exon4 and Insertion of the selection cassette in exon4.
- Disease
Adenocarcinoma
- Concentration
Properties
- Gene name
RNF4
- Gene editing type
Knockout
- Gene editing method
CRISPR technology
- Knockout validation
Sanger Sequencing
Quality control
- STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
EU: 2 US: 2
- Adherent/suspension
Adherent
- Gender
Female
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sanger Sequencing - Human RNF4 knockout HeLa cell lysate (ab259088)
Allele-1: 261 bp deletion in exon4
Sanger Sequencing - Human RNF4 knockout HeLa cell lysate (ab259088)
Allele-2: Insertion of the selection cassette in exon4
Downloads
Product protocols
- Visit the general protocols
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