Human RPE knockout HEK-293T cell lysate
Be the first to review this product! Submit a review
|
(1 Publication)
RPE KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
View Alternative Names
2810429B02Rik, 5730518J08Rik, EC 5.1.3.1, MGC124653, MGC2636, OTTHUMP00000163939, RPE2 1, RPE_HUMAN, Ribulose 5 phosphate epimerase, Ribulose-5-phosphate-3-epimerase, Ribulose-phosphate 3-epimerase
- WB
Lab
Western blot - Human RPE knockout HEK-293T cell lysate (AB258174)
Lane 1 : Wild-type HEK-293T cell lysate, 20 ug
Lane 2 : RPE knockout HEK-293T cell lysate, 20 ug
Lane 3 : HepG2 cell lysate, 20 ug
Lane 4 : A431 cell lysate, 20 ug
False colour image of Western blot : Anti-RPE antibody [EPR7464] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab128891 was shown to bind specifically to RPE. A band was observed at 25 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in rpe knockout cell line ab266325 (knockout cell lysate ab258174). To generate this image, wild-type and rpe knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
false
- Sanger seq
Unknown
Sanger Sequencing - Human RPE knockout HEK-293T cell lysate (AB258174)
Homozygous : 1 bp insertion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Oncology research 32:1173-1184 PubMed38948026
2024
Applications
Unspecified application
Species
Unspecified reactive species
Complementary Products
![Western blot - Anti-RPE antibody [EPR7464] (AB128891)](https://content.abcam.com/products/images/rpe-antibody-epr7464-ab128891--western-blot-img115973.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com