Human RPRM (Protein reprimo) knockout HeLa cell lysate
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RPRM KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 106 bp deletion in exon1 and 53 bp deletion in exon1.
View Alternative Names
RPRM
- Sanger seq
Unknown
Sanger Sequencing - Human RPRM (Protein reprimo) knockout HeLa cell lysate (AB263332)
Allele-1 : 53 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human RPRM (Protein reprimo) knockout HeLa cell lysate (AB263332)
Allele-2 : 106 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RPRM acts as a tumor suppressor by inhibiting cell proliferation mainly through its G2 arrest function. It is not formally known to be part of a larger protein complex but it works closely with p53 a well-known regulatory protein and tumor suppressor to mediate its effects. RPRM expression helps prevent abnormal cell growth under stress conditions contributing to the maintenance of genomic stability.
Pathways
RPRM plays critical roles in the p53 signaling pathway and the cell cycle control pathway. In the p53 pathway RPRM aids in enforcing a cell cycle halt when DNA damage is detected working alongside other proteins such as p21 and GADD45. In the context of cell cycle control RPRM functions as an intermediary that facilitates the arrest of cell division in response to DNA stress signals collaborating with these proteins to enhance DNA repair mechanisms.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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