Human RPS27L knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human RPS27L knockout HeLa cell lysate (AB263333)
Allele-1 : 7 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human RPS27L knockout HeLa cell lysate (AB263333)
Allele-2 : 5 bp deletion in exon2
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein contributes to the regulation of the cellular response to DNA damage and is involved in the control of cell cycle progression. It is part of the small subunit of the ribosome where it associates with other ribosomal proteins to form a functional ribosomal unit. Its activity emphasizes its importance in maintaining genomic stability and proper cellular functions.
Pathways
RPS27L plays a substantial role in the DNA damage response and the p53 signaling pathway. It interacts with the p53 protein an important tumor suppressor regulating p53 levels and activity following genotoxic stress. The interplay between RPS27L and p53 emphasizes its involvement in pathways that oversee damage repair mechanisms particularly by influencing p53-mediated transcriptional regulation and apoptosis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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