Human RQCD1 knockout HeLa cell lysate
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CNOT9 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon1 and 1 bp insertion in exon1 and 68 bp deletion in exon1.
View Alternative Names
CAF40, CCR4-NOT transcription complex subunit 9, CT129, Cell differentiation protein RCD1 homolog, Homo sapiens protein involved in sexual development complete cds, RCD1 required for cell differentiation1 homolog, RCD1 required for cell differentiation1 homolog (S. pombe), RCD1+, RCD1_HUMAN, Rcd-1, Rcd1 (required for cell differentiation, S.pombe) homolog 1
- Sanger seq
Unknown
Sanger Sequencing - Human RQCD1 knockout HeLa cell lysate (AB263334)
Allele-2 : 1 bp insertion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human RQCD1 knockout HeLa cell lysate (AB263334)
Allele-3 : 68 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human RQCD1 knockout HeLa cell lysate (AB263334)
Allele-1 : 16 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein RQCD1 acts as a component of the human mRNA methyltransferase-associated complex. The complex is involved in post-transcriptional modifications. Through its interaction in the complex RQCD1 contributes to RNA processing and stability further affecting gene expression outcomes. This involvement impacts cell cycle regulation and proliferation necessary for maintaining cellular homeostasis.
Pathways
The protein RQCD1 is important in the MAPK signaling pathway and Wnt signaling pathway. In these pathways it interacts with proteins like c-Fos and β-catenin influencing pathways related to cell growth and differentiation. Its presence in these pathways demonstrates the protein's role in signal transduction processes that govern various cellular responses.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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