Human RRAGA (Rag A) knockout HEK-293T cell lysate
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RRAGA KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon1 and 28 bp deletion in exon1 and 2 bp deletion in exon1.
View Alternative Names
Adenovirus E3 14.7 kDa-interacting protein 1, RRAGA_HUMAN, Rag A, Ras-related GTP-binding protein A
- Sanger seq
Unknown
Sanger Sequencing - Human RRAGA (Rag A) knockout HEK-293T cell lysate (AB259090)
Allele-3 : 2 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human RRAGA (Rag A) knockout HEK-293T cell lysate (AB259090)
Allele-2 : 16 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human RRAGA (Rag A) knockout HEK-293T cell lysate (AB259090)
Allele-1 : 28 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Rag A interacts with amino acids and nutrient signaling by activating the mTORC1 complex a central regulator of cell growth. It ensures correct localization of mTORC1 to the lysosomal surface facilitating its activation. The mTORC1 complex includes other proteins such as RAPTOR and mLST8 which collectively modulate cell metabolism and protein synthesis. Rag A's participation is essential for sensing nutrients and the energy state within cells.
Pathways
With regards to pathways Rag A is involved in the mTOR signaling pathway and the autophagy-lysosome pathway. These two pathways are important to maintaining cellular homeostasis and responding to nutrient availability. Rag A directly interacts with Rag C another protein in this pathway to relay the presence of amino acids to mTORC1. Through these interactions Rag A aids in the regulation of cellular growth energy management and metabolism in response to environmental cues.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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