Human RRAGB knockout HeLa cell lysate
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RRAGB KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 85 bp insertion in exon1.
View Alternative Names
GTP-binding protein ragB, RRAGB_HUMAN, Rag B, Ras-related GTP-binding protein B
- Sanger seq
Unknown
Sanger Sequencing - Human RRAGB knockout HeLa cell lysate (AB258644)
Allele-1 : 1 bp insertion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human RRAGB knockout HeLa cell lysate (AB258644)
Allele-2 : 85 bp insertion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RRAGB partners with other Rag GTPases to form heterodimeric complexes. These complexes facilitate the proper localization and activation of mTORC1 on the lysosomal membrane. Moreover RRAGB plays a role in amino acid sensing which impacts cellular growth. By responding to intracellular nutrient levels RRAGB helps modulate anabolic processes and energy balance.
Pathways
RRAGB is integral to the mTOR signaling and nutrient-sensing pathways. As a part of these pathways RRAGB collaborates with proteins such as Raptor and Rheb. These interactions ensure efficient transmission of signals that regulate cell growth and metabolism. The accurate function of this network supports cellular adaptation to varying environmental nutrient conditions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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