Human RRAGC knockout HeLa cell lysate
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RRAGC KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon1 and Insertion of the selection cassette in exon1.
View Alternative Names
AU041672, FLJ13311, GTPase-interacting protein 2, MGC47404, OTTHUMP00000000548, RP23 29H22.4, RRAGC_HUMAN, Rag C, Rag C protein, Ras related GTP binding C, Ras-related GTP-binding protein C, TIB929, YGR163W
- Sanger seq
Unknown
Sanger Sequencing - Human RRAGC knockout HeLa cell lysate (AB258645)
Allele-1 : 2 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human RRAGC knockout HeLa cell lysate (AB258645)
Allele-2 : Insertion of the selection cassette in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RRAGC forms a part of the heterodimeric Rag GTPase complex often teaming with its partner RRAGB. This complex acts as an important regulator in the mTORC1 signaling pathway influencing nutrient sensing and energy balance within cells. RRAGC's ability to toggle between different energy states allows it to contribute to cellular metabolism and response to external stress.
Pathways
RRAGC engages directly in the mTOR signaling pathway an important driver of cell growth and proliferation. It interacts closely with related proteins such as Raptor and mTOR kinase forming a bridge between amino acid availability and cellular growth signals. Additionally RRAGC's role connects to the autophagy pathway affecting cellular degradation processes and nutrient recycling under nutrient-deprived conditions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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