Human RRAS2 (TC21) knockout HEK-293T cell lysate
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RRAS2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 25 bp deletion in exon 11 and 2 bp deletion in exon 11.
View Alternative Names
C86394, Oncogene RRAS2, Oncogene TC21, RRAS2_HUMAN, Ras-like protein TC21, Ras-related protein R-Ras2, Related RAS viral (r ras) oncogene homolog 2, Related RAS viral oncogene homolog 2, Teratocarcinoma oncogene, Teratocarcinoma oncogene TC21
- Sanger seq
Unknown
Sanger Sequencing - Human RRAS2 (TC21) knockout HEK-293T cell lysate (AB259092)
Allele-2 : 2 bp deletion in exon 11
- Sanger seq
Unknown
Sanger Sequencing - Human RRAS2 (TC21) knockout HEK-293T cell lysate (AB259092)
Allele-1 : 25 bp deletion in exon 11
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TC21 influences various cellular processes such as cell growth survival and differentiation. It is active in several signaling pathways contributing to the regulation of cytoskeletal organization and cell adhesion. TC21 is part of larger signaling complexes that engage in mitogenic signaling and influences the function of integrins. These activities position TC21 as a dynamic regulator of cellular behavior allowing it to coordinate and fine-tune responses to extracellular signals.
Pathways
TC21 is integral to the PI3K/AKT and MAPK signaling pathways. In these pathways TC21 interacts with related proteins like PI3K and RAF1 to transmit signals that promote cell proliferation and survival. The PI3K/AKT pathway for instance involves TC21 in the downstream activation of AKT influencing metabolism growth and apoptosis. It also connects with the MAPK pathway contributing to gene expression changes and facilitating cellular responses to growth stimuli.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
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