Human RRBP1 knockout HeLa cell lysate
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RRBP1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 40 bp deletion in exon3 and 41 bp deletion in exon3.
View Alternative Names
180 kDa ribosome receptor homolog, DKFZp586A1420, ES/130, ES/130-related protein, ES130, FLJ36146, KIAA1398, MGC157720, MGC157721, RRBP1_HUMAN, RRP, Ribosome binding protein 1 homolog, Ribosome binding protein 1 homolog 180kDa, Ribosome receptor protein, Ribosome-binding protein 1, hES
- Sanger seq
Unknown
Sanger Sequencing - Human RRBP1 knockout HeLa cell lysate (AB258646)
Allele-1 : 41 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human RRBP1 knockout HeLa cell lysate (AB258646)
Allele-2 : 40 bp deletion in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RRBP1 facilitates the initial binding of ribosomes to the ER membrane a process essential for the proper targeting and translocation of proteins entering the secretory pathway. This protein is integral to the ribosome-ER association acting as a scaffold that stabilizes ribosome attachment during translation. RRBP1 functions as part of a larger translocon complex that includes proteins such as Sec61 which together form a channel for translocating polypeptides into the ER lumen or integrating them into the ER membrane.
Pathways
The protein is essential in protein synthesis and secretion pathways. RRBP1 coordinates with signal recognition particle-dependent pathways where it partners with proteins like Sec61 to ensure accurate translocation of secretory and membrane proteins across the ER membrane. In the unfolded protein response (UPR) pathway RRBP1 might also interact with ER chaperones to assist in protein folding and maintain cellular homeostasis under stress conditions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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