Human RRM2B (p53R2) knockout HeLa cell lysate
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RRM2B KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
View Alternative Names
DKFZp686M05248, MGC102856, MGC42116, MTDPS8A, MTDPS8B, P53 inducible ribonucleotide reductase small subunit 2 short form beta, RIR2B_HUMAN, RRM 2B, Ribonucleoside diphosphate reductase M2 subunit B, Ribonucleoside-diphosphate reductase subunit M2 B, Ribonucleotide reductase M2 B, Ribonucleotide reductase M2 B (TP53 inducible), Ribonucleotide reductase small subunit like 2 p53 inducible, TP53-inducible ribonucleotide reductase M2 B, p53 R2, p53 inducible ribonucleotide reductase small subunit 2 homolog, p53-inducible ribonucleotide reductase small subunit 2-like protein
- WB
Lab
Western blot - Human RRM2B (p53R2) knockout HeLa cell lysate (AB257215)
Lane 1 : Wild-type HeLa cell lysate (40µg)
Lane 2 : RRM2B knockout HeLa cell lysate (40µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab154194 observed at 40 kDa. Red - loading control ab7291 observed at 50 kDa.
ab154194 Rabbit monoclonal [EPR8816] to p53R2 was shown to specifically react with p53R2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261769 (knockout cell lysate ab257215) was used. Wild-type and p53R2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab154194 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-p53R2 antibody [EPR8816] (<a href='/en-us/products/primary-antibodies/p53r2-antibody-epr8816-ab154194'>ab154194</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 40 µg
Lane 2:
RRM2B knockout HeLa cell lysate at 40 µg
Lane 2:
Western blot - Human RRM2B (p53R2) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-rrm2b-p53r2-knockout-hela-cell-line-ab261769'>ab261769</a>)
Predicted band size: 40 kDa
Observed band size: 40 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human RRM2B (p53R2) knockout HeLa cell lysate (AB257215)
Allele-2 : Insertion of the selection cassette in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human RRM2B (p53R2) knockout HeLa cell lysate (AB257215)
Allele-1 : 1 bp deletion in exon 1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
P53R2 plays a role in the synthesis of deoxyribonucleotide triphosphates (dNTPs) for DNA repair. It forms part of a ribonucleotide reductase complex working with other subunits to ensure cells have sufficient dNTP pools for DNA replication and repair. The presence of p53R2 is integral for DNA damage response pathways where it gets activated to supply dNTPs during periods when the p53 tumor suppressor pathway senses DNA damage and activates DNA repair processes.
Pathways
P53R2 is involved in DNA damage response and cell cycle regulation. It interacts with the tumor suppressor protein p53 especially during instances of genotoxic stress facilitating DNA repair and cell cycle arrest to prevent propagation of damaged DNA. Additionally p53R2 relates to proteins such as p21 which are components of the p53 pathway influencing cell cycle arrest and allowing time for DNA repair before cell division resumes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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