Human RRP36 knockout HeLa cell lysate
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RRP36 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon1 and 1 bp insertion in exon1.
View Alternative Names
RRP36_HUMAN, Ribosomal RNA processing protein 36 homolog
- Sanger seq
Unknown
Sanger Sequencing - Human RRP36 knockout HeLa cell lysate (AB263335)
Allele-2 : 1 bp insertion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human RRP36 knockout HeLa cell lysate (AB263335)
Allele-1 : 19 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The RRP36 protein functions as part of the small subunit (SSU) processome a large ribonucleoprotein complex. This complex plays a significant role in the early cleavage steps of pre-rRNA necessary for forming functional ribosomes. By contributing to pre-rRNA processing RRP36 aids in the efficient production of ribosomal RNA a core component of ribosomes. This precise processing is required for ribosome assembly and cellular protein synthesis.
Pathways
RRP36 integrates into the nucleolar small subunit processome-related pathway influential in ribosome biogenesis. This pathway involves many proteins including UTP4 and UTP15 which closely associate with RRP36 in the SSU processome. The successful operation of this pathway is critical for linking ribosome production to overall cellular growth proliferation and metabolism.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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