Human RTN3 (RTN3/HAP) knockout HEK-293T cell lysate
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RTN3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
View Alternative Names
ASYIP, NSP-like protein 2, NSP-like protein II, NSPL2, NSPLII, Neuroendocrine-specific protein-like 2, Neuroendocrine-specific protein-like II, RTN3_HUMAN, Reticulon-3
- WB
Lab
Western blot - Human RTN3 (RTN3/HAP) knockout HEK-293T cell lysate (AB258647)
Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : RTN3 knockout HEK-293T cell lysate 20 μg
Lane 3 : HeLa cell lysate 20 μg
Lane 4 : U-87 MG cell lysate 20 μg
False colour image of Western blot : Anti-RTN3/HAP antibody staining at 1/500 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab68328 was shown to bind specifically to RTN3/HAP. A band was observed at 24 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in RTN3 knockout cell line ab266517 (knockout cell lysate ab258647). To generate this image, wild-type and RTN3 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.
All lanes:
Western blot - Anti-RTN3/HAP antibody (<a href='/en-us/products/primary-antibodies/rtn3-hap-antibody-ab68328'>ab68328</a>) at 1/500 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
RTN3 knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human RTN3 (RTN3/HAP) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-rtn3-rtn3-hap-knockout-hek-293t-cell-line-ab266517'>ab266517</a>)
Lane 3:
HeLa cell lysate at 20 µg
Lane 4:
U-87 MG cell lysate at 20 µg
Predicted band size: 113 kDa
Observed band size: 24 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human RTN3 (RTN3/HAP) knockout HEK-293T cell lysate (AB258647)
Homozygous : 1 bp insertion in exon 1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The RTN3/HAP protein can influence neurite outgrowth implicating it in the development and maintenance of the nervous system. This protein associates closely with other members of the reticulon family forming complexes important for its functional roles within the cell. RTN3/HAP has been implicated in mechanisms that protect neurons from stress especially related to endoplasmic reticulum stress by its participation in membrane remodeling and regulation of lipid metabolism.
Pathways
Data shows that RTN3/HAP interacts with components of the endoplasmic reticulum stress response pathway and lipid biosynthesis pathways. It works alongside proteins such as RTN4/Nogo contributing to the regulation of axon guidance and regeneration. These pathways are essential for maintaining cellular homeostasis and responding to physiological stress with RTN3/HAP helping adapt or protect cells under challenging conditions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Cell Lines & Lysates
AB266517
Human RTN3 (RTN3/HAP) knockout HEK-293T cell line
Advanced Validation
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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