Human RTN4RL2 (NgR2) knockout HeLa cell lysate
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RTN4RL2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon2.
View Alternative Names
NGRH1, NGRL3, NgR2, Nogo receptor-like 3, Nogo-66 receptor homolog 1, Nogo-66 receptor-related protein 2, R4RL2_HUMAN, RTN4RL2, Reticulon-4 receptor-like 2
- Sanger seq
Unknown
Sanger Sequencing - Human RTN4RL2 (NgR2) knockout HeLa cell lysate (AB263336)
Homozygous : 10 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The action of NgR2 impacts the neural regeneration processes by forming a receptor complex with p75 neurotrophin receptor (p75NTR) and LINGO-1. This complex activation inhibits axonal outgrowth and neural plasticity which affects the nervous system's ability to recover after injury. NgR2 mediates signaling that prevents neurite outgrowth contributing to the stabilization of neuronal connections in postnatal brain development. NgR2's role in these processes makes it a significant target for studies investigating neuronal repair mechanisms.
Pathways
Axon guidance and myelin inhibition play significant roles in the function of NgR2. It participates in the RhoA-ROCK signaling pathway which is activated upon myelin ligand binding to the receptor complex. This pathway is responsible for cytoskeletal changes that inhibit neurite extension. Additionally NgR2 interacts with proteins such as Nogo-A and MAG further influencing axonal growth inhibition and regeneration in the nervous system.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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