Human RTP4 knockout A549 cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human RTP4 knockout A549 cell lysate (AB259094)
Homozygous : 5 bp deletion in exon2
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The RTP4 protein aids in the transport and functional expression of GPCRs without forming part of any known stable complexes. This chaperone activity is vital for GPCR function impacting signal transduction by promoting proper receptor folding and surface expression. RTP4 ensures that olfactory and other related receptors can adequately respond to stimuli. Its chaperone activity is important for maintaining GPCR dynamics in various cell types.
Pathways
RTP4 significantly influences GPCR signaling pathways and olfactory signaling pathways. It directly affects the transport and activation of olfactory receptors key components in the olfactory signaling pathway. RTP4's interaction with olfactory receptors impacts the downstream components such as adenylyl cyclase leading to successful signal transduction. Additionally RTP4 interacts with proteins in GPCR signaling that modify receptor availability on the cell surface.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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