Human RYK knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human RYK knockout HeLa cell lysate (AB263337)
Allele-1 : 1 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human RYK knockout HeLa cell lysate (AB263337)
Allele-2 : 160 bp deletion in exon2
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RYK modulates cellular communication by interacting with Wnt proteins. It does not possess intrinsic kinase activity but participates in signaling as part of a larger receptor complex on the cell surface. This complex includes other proteins such as Frizzled receptors and LRP co-receptors facilitating the translation of extracellular Wnt signals into intracellular responses. RYK although not a conventional kinase plays a pivotal role in neuronal development influencing axon guidance and synaptic formation.
Pathways
RYK is integral to the Wnt signaling pathway which is essential for cell proliferation differentiation and migration. Through the Wnt pathway RYK interacts with other proteins such as Disheveled (Dvl) to execute its signaling functions. Additionally RYK has associations with the non-canonical Wnt/PCP (planar cell polarity) pathway modulating tissue polarity and cell movement during embryogenesis. Its role within these pathways highlights the intricate regulatory networks involved in developmental biology.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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