Human S100A11 knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
S100A11 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.
View Alternative Names
S100C, MLN 70, Metastatic lymph node gene 70 protein, S100 calcium-binding protein A11, Protein S100-C, S100A11, Protein S100-A11, MLN70, Calgizzarin
- Sanger seq
Unknown
Sanger Sequencing - Human S100A11 knockout HeLa cell lysate (AB257651)
Homozygous : 1 bp insertion in exon2
- WB
Lab
Western blot - Human S100A11 knockout HeLa cell lysate (AB257651)
Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : S100A11 knockout HeLa cell lysate 20 μg
Lane 3 : U-2 OS cell lysate 20 μg
Lane 4 : PC-3 cell lysate 20 μg
False colour image of Western blot : Anti-S100A11 antibody (ab187180) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab187180 was shown to bind specifically to S100A11. A band was observed at 12 kDa in wild-type HeLa cell lysates with no signal observed at this size in S100A11 knockout cell line ab265085 (knockout cell lysate ab257651). To generate this image, wild-type and S100A11 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Anti-S100A11 antibody (<a href='/en-us/products/unavailable/s100a11-antibody-ab187180'>ab187180</a>) at 1/1000 dilution
Lane 1:
HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human S100A11 knockout HeLa cell lysate (ab257651) at 1/20000 dilution
Lane 3:
U-2 OS cell lysate at 20 µg
Lane 4:
PC-3 cell lysate at 20 µg
Secondary
Lanes 1 - 4:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Lanes 1 - 4:
Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution
Observed band size: 36 kDa
false
Complementary Products
Primary Antibodies
AB180593
Anti-S100A11 antibody [EPR11172]
20ul selling size|RabMAb|Recombinant
![Flow Cytometry (Intracellular) - Anti-S100A11 antibody [EPR11172] (AB180593)](https://content.abcam.com/products/images/s100a11-antibody-epr11172-ab180593--flow-cytometry-intracellular-img84989.jpg)
- 5
- 2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The functions of S100A11 include involvement in cell proliferation differentiation and migration. S100A11 often operates as part of a protein complex facilitating these cellular processes by interacting with other signaling molecules. It can shuttle between the cytoplasm and nucleus influencing gene expression and cellular dynamics. S100A11 has also shown involvement in smooth muscle contraction and skin barrier function regulation.
Pathways
The activity involving S100A11 spans multiple biological pathways including the calcium signaling pathway and the epithelial cell signaling pathway. Through these pathways S100A11 maintains critical cellular homeostasis and function. In the calcium signaling pathway it interacts with proteins like annexins and calcyclin to modulate cellular responses to calcium influx. In epithelial cell signaling it affects pathways related to cell adhesion and communication integrating signals that dictate cell behavior in response to external stimuli.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com