Human S100A16 knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human S100A16 knockout HeLa cell lysate (AB259095)
Allele-1 : 1 bp insertion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human S100A16 knockout HeLa cell lysate (AB259095)
Allele-2 : 4 bp insertion in exon2
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Quality control - STR analysis
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
S100A16 is involved in cellular processes such as proliferation differentiation and migration. It participates in the modulation of cytoskeletal dynamics and cell cycle regulation. S100A16 often integrates into multiprotein complexes where it interacts with target proteins to exert its biological effects. Studies show that S100A16 may also contribute to epithelial-mesenchymal transition (EMT) an important event in development and cancer progression.
Pathways
S100A16 functions in signaling pathways linked to calcium-dependent processes. In the MAPK signaling pathway it interacts with proteins like ERK1/2 to regulate cell proliferation and survival. Furthermore S100A16 is also associated with the TGF-beta pathway where its role in EMT becomes significant and involves interactions with TGF-beta-related factors. These pathways underline the importance of S100A16 in cellular homeostasis and developmental processes.
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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