Human S100P knockout HeLa cell lysate
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S100P KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
View Alternative Names
MIG9, S100P, Protein S100-E, Protein S100-P, S100E, Migration-inducing gene 9 protein, S100 calcium-binding protein P
- WB
Lab
Western blot - Human S100P knockout HeLa cell lysate (AB257653)
Lane 1 : Wild-type HeLa cell lysate 40 μg
Lane 2 : S100P knockout HeLa cell lysate 40 μg
Lane 3 : HepG2 cell lysate 40 μg
Lane 4 : SW480 cell lysate 40 μgFalse colour image of Western blot : Anti-S100P antibody [EPR6142] (ab124743) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red.
In Western blot, ab124743 was shown to bind specifically to S100P. A band was observed at 130 kDa in wild-type HeLa cell lysates with no signal observed at this size in S100P knockout cell line ab266026 (knockout cell lysate ab257653). To generate this image, wild-type and S100P knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent Western blot blocking reagent in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat Anti-Rabbit IgG H&L (HRP) (ab6721) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-S100P antibody [EPR6142] (<a href='/en-us/products/primary-antibodies/s100p-antibody-epr6142-ab124743'>ab124743</a>)
Predicted band size: 10 kDa
Observed band size: 9 kDa
false
Exposure time: 20min
- Sanger seq
Unknown
Sanger Sequencing - Human S100P knockout HeLa cell lysate (AB257653)
Allele-2 : Insertion of the selection cassette in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human S100P knockout HeLa cell lysate (AB257653)
Allele-1 : 1 bp deletion in exon 1
Complementary Products
Primary Antibodies
AB124743
20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6142] (AB124743)](https://content.abcam.com/products/images/s100p-antibody-epr6142-ab124743--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img85062.jpg)
- 0
- 0
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
S100P plays a role in intracellular and extracellular calcium signaling. It influences cell survival proliferation and migration. It can function as a monomer but often interacts with other proteins forming complexes such as with RAGE (Receptor for Advanced Glycation End-products) which amplifies signaling cascades that control cellular responses. Its binding to calcium and interaction with RAGE modulate important cellular processes tied to cell cycle and cellular stress response.
Pathways
S100P is closely involved in the MAPK (Mitogen-Activated Protein Kinases) and RAGE signaling pathways. Its interaction with RAGE leads to activation of the MAPK pathway facilitating cellular responses such as growth and survival. S100P also coordinates with other S100 proteins such as S100A4 and S100A9 enhancing its involvement in regulating cellular functions through these pathways. These interactions highlight the importance of S100P in cellular communication and response mechanisms.
Quality control
STR analysis
D5S818, TH01, D16S539, TPOX, CSF1PO, D13S317, D7S820
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com