Human S1PR1 (S1P1/EDG1) knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human S1PR1 (S1P1/EDG1) knockout HeLa cell lysate (AB257654)
Allele-2 : 1 bp insertion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human S1PR1 (S1P1/EDG1) knockout HeLa cell lysate (AB257654)
Allele-1 : 1 bp deletion in exon2
Complementary Products
Primary Antibodies
AB302714
Anti-SPHK1 antibody [EPR24511-37]
20ul selling size|RabMAb|Recombinant|KO Validated
![Western blot - Anti-SPHK1 antibody [EPR24511-37] (AB302714)](https://content.abcam.com/products/images/sphk1-antibody-epr24511-37-ab302714--western-blot-img401143.jpg)
- 0
- 0
![Immunocytochemistry/ Immunofluorescence - Anti-S1P1/EDG1 antibody [EPR21202] (AB233386)](https://content.abcam.com/products/images/s1p1-edg1-antibody-epr21202-ab233386--immunocytochemistry-immunofluorescence-img430408.jpg)
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
S1P1 is involved in the regulation of immune cell trafficking and vascular stability. As a part of a receptor complex it activates intracellular signaling cascades that influence cell migration and vascular maturation. By binding to S1P S1P1 controls the egress of lymphocytes from lymphoid tissues indicating its important role in immune surveillance and response.
Pathways
S1P1 serves as an important component in the S1P signaling pathway and the phosphoinositide 3-kinase (PI3K) pathway. In the S1P pathway it regulates endothelial cell barrier function and vascular maturation. In the PI3K pathway S1P1 influences cell survival and migration which relates it to protein kinases like Akt and other signaling molecules that further modify cellular responses.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com