Human SAA1 knockout A549 cell lysate
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SAA1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 137 bp insertion in exon3 and 1 bp insertion in exon3.
View Alternative Names
Amyloid fibril protein AA, Amyloid protein A, MGC111216, PIG 4, Serum amyloid A 1, Serum amyloid A protein, TP53I4, Tumor protein p53 inducible protein 4, amyloid A, serum, serum amyloid A-1 protein
- Sanger seq
Unknown
Sanger Sequencing - Human SAA1 knockout A549 cell lysate (AB257102)
Allele-1 : 137 bp insertion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human SAA1 knockout A549 cell lysate (AB257102)
Allele-2 : 1 bp insertion in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein regulates cholesterol metabolism and serves as a chemoattractant directing cell movement towards the sites of inflammation. SAA1 does not exist alone often associating with high-density lipoproteins (HDL) in circulation. By binding to HDL it can aid in transporting cholesterol from tissues to the liver for excretion linking it with both immunological and metabolic processes.
Pathways
Researchers place SAA1 within the inflammation and immune response strategies. It significantly contributes to the acute-phase response and relates to cytokine signaling pathways. SAA1 interacts with proteins such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) which are central to the activation and propagation of inflammatory responses in tissues.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Primary Antibodies
AB190802
Anti-Serum Amyloid A antibody [EP11592-92]
BOND RX™ Validated|RabMAb|Recombinant|Trial Size
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Amyloid A antibody [EP11592-92] (AB190802)](https://content.abcam.com/products/images/serum-amyloid-a-antibody-ep11592-92-ab190802--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132293.jpg)
- 0
- 1
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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