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AB257656

Human SAMD9 knockout A549 cell lysate

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SAMD9 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon2.
2 Images
Western blot - Human SAMD9 knockout A549 cell lysate (AB257656)
  • WB

Lab

Western blot - Human SAMD9 knockout A549 cell lysate (AB257656)

Lane 1 : Wild-type A549 cell lysate (20 ug)
Lane 2 : SAMD9 knockout A549 cell lysate (20 ug)
Lane 3 : A431 cell lysate (20 ug)
Lane 4 : Human skeletal muscle cell lysate (20 ug)

ab180575 was shown to specifically react with SAMD9 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267038 (knockout cell lysate ab257656) was used. Wild-type and SAMD9 knockout samples were subjected to SDS-PAGE. ab180575 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SAMD9 antibody [EPR13603] (<a href='/en-us/products/primary-antibodies/samd9-antibody-epr13603-ab180575'>ab180575</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

SAMD9 knockout A549 cell lysate at 20 µg

Lane 2:

Western blot - Human SAMD9 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-samd9-knockout-a549-cell-line-ab267038'>ab267038</a>)

Lane 3:

A431 cell lysate at 20 µg

Lane 4:

Human skeletal muscle cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 184 kDa

Observed band size: 184 kDa

false

Sanger Sequencing - Human SAMD9 knockout A549 cell lysate (AB257656)
  • Sanger seq

Unknown

Sanger Sequencing - Human SAMD9 knockout A549 cell lysate (AB257656)

Homozygous : 1 bp insertion in exon2

Key facts

Cell type

A549

Species or organism

Human

Tissue

Lung

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon2.

Disease

Carcinoma

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Complementary Products

Primary Antibodies

AB180575

Anti-SAMD9 antibody [EPR13603]

RabMAb|Recombinant|KO Validated

Western blot - Anti-SAMD9 antibody [EPR13603] (AB180575)

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  • 0
Proteins & Peptides

AB259412

Recombinant human VEGF 165A protein (Active)

Premium Bioactive

Mass Spectrometry - Recombinant human VEGF 165A protein (Active) (AB259412)

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Proteins & Peptides

AB288799

Recombinant Human VCAM1 protein (Active)

Premium Bioactive

Mass Spectrometry - Recombinant Human VCAM1 protein (Active) (AB288799)

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Cell Lines & Lysates

AB267039

Human SAMD9 knockout A549 cell line

Advanced Validation

Western blot - Human SAMD9 knockout A549 cell line (AB267039)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
SAMD9
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SAMD9 also known as sterile alpha motif domain-containing 9 is an intriguing protein with a molecular mass of approximately 180 kDa. Predominantly expressed in tissues such as bone marrow and lymph nodes it plays a role in cellular proliferation and response to stress stimuli. The protein is part of the larger family of SAMD proteins which contain sterile alpha motifs influencing protein-protein interactions and it engages in regulation steps at a molecular level.
Biological function summary

SAMD9 takes part in controlling cellular proliferation and inhibiting abnormal growth mostly acting as a growth suppressor. Its functions link to maintaining homeostasis within cells and aiding in preventing uncontrolled cell expansion. SAMD9 functions independently of any known stable protein complex performing its regulatory role in diverse cellular contexts. The protein’s involvement spans multiple cell types reflecting its broad impact on normal human physiology.

Pathways

SAMD9 interacts with critical signaling modules such as the mTOR pathway and the interferon signaling pathway. It modulates cellular responses related to growth control and stress adaptation. Interactions with other proteins like TSC1 and TSC2 within the mTOR pathway highlight SAMD9's engagement in regulating cellular responses to environmental changes and nutrient availability.

SAMD9 is associated with severe conditions like myelodysplastic syndromes and MIRAGE syndrome. These conditions link to mutations affecting the protein's regulatory capacities leading to disrupted cellular processes and disease progression. The protein shares connections with SAMD9L another related protein indicating a potential overlap in disease mechanisms and impacts. Understanding the behavior of SAMD9 in these contexts is important for developing therapeutic strategies and improving disease management.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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