Human SAMD9L knockout A549 cell lysate
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SAMD9L KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon5 and 1 bp deletion in exon5.
View Alternative Names
AA175286, C7orf6, DRIF2, ESTM25, FLJ39885, KIAA2005, OTTHUMP00000208675, OTTHUMP00000208676, OTTHUMP00000208677, OTTHUMP00000208678, OTTHUMP00000208679, OTTHUMP00000208680, OTTMUSP00000038123, OTTMUSP00000038131, SAM domain containing protein 9 like, Sterile alpha motif domain containing 9 like, Sterile alpha motif domain containing protein 9 like, UEF, UEF1, V myc myelocytomatosis viral related oncogene, neuroblastoma derived, mKIAA2005
- Sanger seq
Unknown
Sanger Sequencing - Human SAMD9L knockout A549 cell lysate (AB258648)
Allele-2 : 1 bp deletion in exon5
- Sanger seq
Unknown
Sanger Sequencing - Human SAMD9L knockout A549 cell lysate (AB258648)
Allele-1 : 11 bp deletion in exon5
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The SAMD9L protein is involved in important cellular processes and hematopoiesis. Through its role in regulating immune responses and cell growth it interacts with other proteins to form functional complexes that facilitate homeostasis in the body. Its presence impacts how cells react to external stress and signals affecting both normal and pathological conditions.
Pathways
SAMD9L interacts with elements of the innate immune system and the cellular stress response pathways. It engages with the JAK-STAT signaling pathway affecting transcriptional activity linked to cell proliferation and survival. SAMD9L also shows interactions with proteins like STAT1 and STAT3 influencing signal transduction processes that maintain cell equilibrium under stress conditions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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