SAMD9L KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon5 and 1 bp insertion in exon5.
Images
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon5 and 1 bp insertion in exon5.
Alternative names
AA175286, C7orf6, DRIF2, ESTM25, FLJ39885, KIAA2005, OTTHUMP00000208675, OTTHUMP00000208676, OTTHUMP00000208677, OTTHUMP00000208678, OTTHUMP00000208679, OTTHUMP00000208680, OTTMUSP00000038123, OTTMUSP00000038131, SAM domain containing protein 9 like, Sterile alpha motif domain containing 9 like, Sterile alpha motif domain containing protein 9 like, UEF, UEF1, V myc myelocytomatosis viral related oncogene, neuroblastoma derived, mKIAA2005
What's included?
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SAMD9L KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon5 and 1 bp insertion in exon5.
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon5 and 1 bp insertion in exon5.
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- SAMD9L
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SAMD9L also known as Sterile Alpha Motif Domain-Containing Protein 9-Like plays a significant role in regulating cellular proliferation. This protein weighs approximately 181 kDa and is expressed in several tissues with higher levels observed in the bone marrow and lymphoid tissues. SAMD9L functions mechanically by modulating cellular responses to growth signals thereby influencing various cellular activities.
- Biological function summary
The SAMD9L protein is involved in important cellular processes and hematopoiesis. Through its role in regulating immune responses and cell growth it interacts with other proteins to form functional complexes that facilitate homeostasis in the body. Its presence impacts how cells react to external stress and signals affecting both normal and pathological conditions.
- Pathways
SAMD9L interacts with elements of the innate immune system and the cellular stress response pathways. It engages with the JAK-STAT signaling pathway affecting transcriptional activity linked to cell proliferation and survival. SAMD9L also shows interactions with proteins like STAT1 and STAT3 influencing signal transduction processes that maintain cell equilibrium under stress conditions.
- Associated diseases and disorders
Researchers have linked SAMD9L to myelodysplastic syndromes and hematopoietic malignancies. Mutations or dysregulations of SAMD9L can lead to aberrant cell growth and differentiation contributing to disease progression. It is associated with other molecules such as interferon-alpha which can modulate the inflammatory response in these conditions providing specific targets for diagnostic and therapeutic approaches.
Product promise
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Terms & Conditions.
2 product images
Sanger Sequencing - Human SAMD9L knockout A549 cell lysate (ab258649)
Allele-2: 11 bp deletion in exon5
Sanger Sequencing - Human SAMD9L knockout A549 cell lysate (ab258649)
Allele-1: 1 bp insertion in exon5
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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