Human SCAF11 (SFRS2IP) knockout HeLa cell lysate
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SCAF11 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon10 and 2 bp deletion in exon10.
View Alternative Names
1110061H03Rik, 2610510E10Rik, AI462454, CASP-11, CTD associated SR protein 11, Renal carcinoma antigen NY REN 40, SC35 interacting protein 1, SFRS2IP, SIP-1, SRrp129, Splicing regulatory protein 129, mKIAA3013, splicing factor, arginine/serine rich 2, interacting protein
- Sanger seq
Unknown
Sanger Sequencing - Human SCAF11 (SFRS2IP) knockout HeLa cell lysate (AB259097)
Allele-1 : 2 bp deletion in exon10
- Sanger seq
Unknown
Sanger Sequencing - Human SCAF11 (SFRS2IP) knockout HeLa cell lysate (AB259097)
Allele-2 : 1 bp deletion in exon10
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Proteins like SFRS2IP contribute to the regulation of RNA processing and stability. SFRS2IP participates in the spliceosome complex facilitating correct splicing and maturation of precursor mRNA into mature RNA. This process is essential for gene expression regulation ensuring that proteins are synthesized properly and in necessary amounts. SFRS2IP's involvement in these processes influences cell cycle progression and can impact cellular responses to environmental changes.
Pathways
Several studies have included SFRS2IP in processes related to mRNA splicing and gene expression. It is closely connected to the spliceosome pathway where it cooperates with SFRS2 and other splicing factors contributing to proper intron removal and exon joining in precursor mRNA. The protein also interacts with components of the RNA transport pathway ensuring the correct delivery of mRNA to the ribosome for translation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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