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AB258184

Human SCAMP1 knockout HeLa cell lysate

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SCAMP1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 25 bp deletion in exon2.
4 Images
Western blot - Human SCAMP1 knockout HeLa cell lysate (AB258184)
  • WB

Lab

Western blot - Human SCAMP1 knockout HeLa cell lysate (AB258184)

Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : SCAMP1 knockout HeLa cell lysate 20 μg

Lanes 1 - 2 : Merged signal (red and green). Green - Anti-SCAMP1 antibody observed at 36 kDa. Red - loading control, ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa. Anti-SCAMP1 antibody was shown to react with SCAMP1 in wild-type HeLa cells in western blot. The bands observed in SCAMP1 knockout cell line ab265567 (SCAMP1 knockout cell lysate ab258184) below 36 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. HeLa wild-type and SCAMP1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with anti-SCAMP1 antibody and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 5 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

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Sanger Sequencing - Human SCAMP1 knockout HeLa cell lysate (AB258184)
  • Sanger seq

Unknown

Sanger Sequencing - Human SCAMP1 knockout HeLa cell lysate (AB258184)

Allele-1 : 25 bp deletion in exon2

Sanger Sequencing - Human SCAMP1 knockout HeLa cell lysate (AB258184)
  • Sanger seq

Unknown

Sanger Sequencing - Human SCAMP1 knockout HeLa cell lysate (AB258184)

Allele-2 : 1 bp insertion in exon2

Western blot - Human SCAMP1 knockout HeLa cell lysate (AB258184)
  • WB

Supplier Data

Western blot - Human SCAMP1 knockout HeLa cell lysate (AB258184)

Lanes 1 - 2 : Merged signal (red and green). Green - ab3430 observed at 36 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa. ab3430 was shown to react with SCAMP1 in wild-type HeLa cells in western blot. The bands observed in SCAMP1 knockout cell line ab265567 (SCAMP1 knockout cell lysate ab258184) below 36 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. HeLa wild-type and SCAMP1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab3430 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at 1 µg/ml and a 1/20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-SCAMP1 antibody (<a href='/en-us/products/primary-antibodies/scamp1-antibody-ab3430'>ab3430</a>) at 1 µg/mL

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human SCAMP1 knockout HeLa cell lysate (ab258184) at 20 µg

Secondary

Lanes 1 - 2:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution

Lanes 1 - 2:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 38 kDa

Observed band size: 36 kDa

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Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 25 bp deletion in exon2.

Disease

Adenocarcinoma

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Complementary Products

Primary Antibodies

AB3430

Anti-SCAMP1 antibody

Western blot - Anti-SCAMP1 antibody (AB3430)

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  • 0
Cell Lines & Lysates

AB265567

Human SCAMP1 knockout HeLa cell line

Advanced Validation

Western blot - Human SCAMP1 knockout HeLa cell line (AB265567)

  • 0
  • 0
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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
SCAMP1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCAMP1 short for Secretory Carrier Membrane Proteins 1 plays a mechanical role in the transport and recycling of membranes. It is a protein of approximately 38 kDa and typically localizes to the vesicular membranes within the cytoplasm. Expression of SCAMP1 can be observed in a variety of tissues including those of the nervous system and endocrine glands reflecting its involvement in intricate vesicular traffic processes.
Biological function summary

SCAMP1 is engaged in the regulation of endocytosis and exocytosis within cellular processes. It is not part of a conventional complex but interacts dynamically with other proteins involved in vesicle fusion and release. SCAMP1 associates with cytosolic domains and membrane proteins to orchestrate the trafficking of vesicles ensuring efficient cellular communication and molecular transport across cellular membranes.

Pathways

Proteins related to vesicular transport such as SNAREs interact functionally with SCAMP1. Within this context SCAMP1 is actively involved in the endocytic and exocytic pathways which are important for maintaining cellular homeostasis and neurotransmitter release. These pathways are vital for the release of neurotransmitters during synaptic signaling and hormone secretion in endocrine systems with SCAMP1 aiding in the precise regulation of these events.

Dysregulation of SCAMP1 associates with neurodegenerative conditions like Alzheimer's disease and metabolic disorders such as diabetes mellitus. SCAMP1's connection to SNARE proteins in these scenarios highlights its impactful role in synaptic and endocrine dysfunctions. In Alzheimer's disease altered vesicle recycling and transport influenced by SCAMP1 may contribute to impaired neuronal signaling pathways while in diabetes it affects insulin secretion dynamics important for glucose metabolism.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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