Human SCAND1 knockout HeLa cell lysate
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SCAND1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and Insertion of the selection cassette in exon2.
View Alternative Names
Leap1, Leucine rich domain interacting protein 1, PPAR gamma cofactor 2, Pgc2, Ppargc2, RAZ1, SCAN domain containing 1, SCAN domain-containing protein 1, SCAN related protein RAZ1, SCND1_HUMAN, SDP1
- Sanger seq
Unknown
Sanger Sequencing - Human SCAND1 knockout HeLa cell lysate (AB263338)
Allele-2 : Insertion of the selection cassette in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human SCAND1 knockout HeLa cell lysate (AB263338)
Allele-1 : 1 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SCAND1 acts as a coordinator in cellular transcription machinery by interacting with other proteins. It often forms part of multiprotein complexes that govern chromatin remodeling and gene transcription. Through its interactions SCAND1 can influence the expression of genes that are critical in cell cycle regulation and development. These interactions contribute to cellular homeostasis and equilibrium.
Pathways
SCAND1 fits into gene transcription and chromatin remodeling circuits. Specifically it engages in interactions within the Notch signaling pathway and the Wnt signaling pathway. These pathways involve cooperation with other proteins like Notch receptors and beta-catenin which are pivotal in developmental processes and cell fate determination. SCAND1 modulates transcriptional responses that are delicate to environmental and intracellular cues.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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