SCARB1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 40 bp deletion in exon 2.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 40 bp deletion in exon 2
Alternative names
CD36 and LIMPII analogous 1, CD36 antigen-like 1, CD36L1, CLA-1, Collagen type I receptor, HDLQTL6, MGC138242, SCARB1, SCRB1_HUMAN, SR-BI, SRB1, Scavebger Receptor Class B Member 1, Scavenger Receptor Class B Type 1, Scavenger receptor class B member 1, thrombospondin receptor-like 1
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SCARB1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 40 bp deletion in exon 2.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 40 bp deletion in exon 2
- Concentration
Properties
- Gene name
- SCARB1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing, Western blot
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Scavenging Receptor Class B Type 1 (SR-BI) also known as CLA-1 in humans is a membrane protein involved in the selective uptake of lipids. This protein has a molecular mass of approximately 82 kDa and is primarily expressed in the liver and non-placental tissues such as adrenal glands and ovaries. SR-BI mediates the transfer of cholesterol esters from high-density lipoprotein (HDL) to cells while playing a role in reverse cholesterol transport. Its function is essential for maintaining cholesterol homeostasis.
- Biological function summary
SR-BI contributes to lipid metabolism by influencing the dynamics of cholesterol and lipid circulation within the body. It forms part of the larger HDL receptor complex which facilitates the selective uptake of lipids without internalizing the entire lipoprotein. Through this mechanism SR-BI assists cells in acquiring necessary components for membrane synthesis and hormone production.
- Pathways
SR-BI integrates into the cholesterol biosynthesis pathway and the reverse cholesterol transport pathway. In these pathways SR-BI works closely with proteins like ATP-binding cassette transporter A1 (ABCA1) and lecithin-cholesterol acyltransferase (LCAT). These interactions promote the efflux and transport of cholesterol therefore playing a vital role in lipid regulation and metabolic processes.
- Associated diseases and disorders
SR-BI's malfunction can lead to cardiovascular diseases due to its role in cholesterol metabolism. Elevated levels of HDL cholesterol without functional SR-BI impair reverse cholesterol transport potentially leading to atherosclerosis. Moreover SR-BI interacts with apolipoprotein A-I (apoA-I) a major protein component of HDL impacting its efficacy and connection to coronary artery disease. Understanding SR-BI pathways and their interactions can provide insights into therapeutic strategies for treating cholesterol-related disorders.
Product promise
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4 product images
Western blot - Human SCARB1 knockout HEK-293T cell lysate (ab283046)
Lane 1: Wild-type HEK-293T cell lysate, 20 μg.
Lane 2: SCARB1 knockout HEK-293T cell lysate, 20 μg.
Lane 3: Human Liver cell lysate, 20 μg.False colour image of Western blot: Anti-Scavenging Receptor SR-BI antibody [EPR20190] staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Scavenging Receptor SR-BI antibody [EPR20190] ab217318 was shown to bind specifically to Scavenging Receptor SR-BI. A band was observed at 70/75 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in SCARB1 knockout cell line Human SCARB1 knockout HEK-293T cell line ab282646 (knockout cell lysate ab283046). To generate this image, wild-type and SCARB1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (Anti-Scavenging Receptor SR-BI antibody [EPR20190] ab217318) at 1/2000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: SCARB1 knockout HEK-293T cell lysate at 20 µg
Lane 2: Western blot - Human SCARB1 knockout HEK-293T cell line (Human SCARB1 knockout HEK-293T cell line ab282646)
Lane 3: Human Liver cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 70 kDa, 75 kDa
Western blot - Human SCARB1 knockout HEK-293T cell lysate (ab283046)
Lane 1: Wild-type HEK-293T cell lysate, 20 μg.
Lane 2: SCARB1 knockout HEK-293T cell lysate, 20 μg.
Lane 3: Human Liver cell lysate, 20 μg.False colour image of Western blot: Anti-Scavenging Receptor SR-BI antibody [EP1556Y] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Scavenging Receptor SR-BI antibody [EP1556Y] ab52629 was shown to bind specifically to Scavenging Receptor SR-BI. A band was observed at 70/75 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in SCARB1 knockout cell line Human SCARB1 knockout HEK-293T cell line ab282646 (knockout cell lysate ab283046). To generate this image, wild-type and SCARB1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (Anti-Scavenging Receptor SR-BI antibody [EP1556Y] ab52629) at 1/1000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: SCARB1 knockout HEK-293T cell lysate at 20 µg
Lane 2: Western blot - Human SCARB1 knockout HEK-293T cell line (Human SCARB1 knockout HEK-293T cell line ab282646)
Lane 3: Human Liver cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 70 kDa, 75 kDa
Sanger Sequencing - Human SCARB1 knockout HEK-293T cell lysate (ab283046)
40 bp deletion in exon 2
Western blot - Human SCARB1 knockout HEK-293T cell lysate (ab283046)
False colour image of Western blot: Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] staining at 1/1000 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] ab300632 was shown to bind specifically to Scavenging Receptor SR-BI. A band was observed at 70 and 75 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in SCARB1 knockout cell line Human SCARB1 knockout HEK-293T cell line ab282646. To generate this image, wild-type and SCARB1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/20000 dilution.
All lanes: Western blot - Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] (Anti-Scavenging Receptor SR-BI antibody [25/CLA-1] ab300632) at 1/1000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: Western blot - Human SCARB1 knockout HEK-293T cell lysate (ab283046)
Lane 2: Western blot - Human SCARB1 knockout HEK-293T cell line (Human SCARB1 knockout HEK-293T cell line ab282646)
Lane 2: SCARB1 knockout HEK-293T cell lysate at 20 µg
Lane 3: Human Liver cell lysate at 20 µg
SecondaryLanes 1 - 3: Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/20000 dilution
Lanes 1 - 3: Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 70 kDa, 75 kDa
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