Human SCARB2 knockout MCF7 cell lysate

• WB

Lab

Western blot - Human SCARB2 knockout MCF7 cell lysate (AB275010)

Lane 1 : Wild-type MCF7 cell lysate 20 μg
Lane 2 : SCARB2 knockout MCF7 cell lysate 20 μg
False colour image of Western blot : Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal staining at 1/20000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab196651 was shown to bind specifically to Scavenging Receptor SRB2. A band was observed at 80 kDa in wild-type MCF7 cell lysates with no signal observed at this size in SCARB2 knockout cell line ab274952 (knockout cell lysate ab275010). To generate this image, wild-type and SCARB2 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (<a href='/en-us/products/primary-antibodies/scavenging-receptor-srb2-antibody-epr12081-c-terminal-ab196651'>ab196651</a>) at 1/20000 dilution

Lane 1:

Wild-type MCF7 cell lysate at 20 µg

Lane 2:

SCARB2 knockout MCF7 cell lysate at 20 µg

Lane 2:

Western blot - Human SCARB2 knockout MCF7 cell line (<a href='/en-us/products/cell-lines/human-scarb2-knockout-mcf7-cell-line-ab274952'>ab274952</a>)

Predicted band size: 54 kDa

Observed band size: 80 kDa

false

• WB

Lab

Western blot - Human SCARB2 knockout MCF7 cell lysate (AB275010)

Lane 1 : Wild-type MCF7 cell lysate 20 μg
Lane 2 : SCARB2 knockout MCF7 cell lysate 20 μg
Lane 3 : SH-SY5Y cell lysate 20 μg
Lane 4 : HEK-293 cell lysate 20 μg
False colour image of Western blot : Anti-LIMPII antibody [EPR12080] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab176317 was shown to bind specifically to LIMPII. A band was observed at 80 kDa in wild-type MCF7 cell lysates with no signal observed at this size in SCARB2 knockout cell line ab274952 (knockout cell lysate ab275010). To generate this image, wild-type and SCARB2 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-LIMPII antibody [EPR12080] (<a href='/en-us/products/primary-antibodies/limpii-antibody-epr12080-ab176317'>ab176317</a>) at 1/1000 dilution

Lane 1:

Wild-type MCF7 cell lysate at 20 µg

Lane 2:

SCARB2 knockout MCF7 cell lysate at 20 µg

Lane 2:

Western blot - Human SCARB2 knockout MCF7 cell line (<a href='/en-us/products/cell-lines/human-scarb2-knockout-mcf7-cell-line-ab274952'>ab274952</a>)

Lane 3:

SH-SY5Y cell lysate at 20 µg

Lane 4:

HEK-293 cell lysate at 20 µg

Predicted band size: 54 kDa

Observed band size: 80 kDa

false

• NGS

Supplier Data

Next Generation Sequencing - Human SCARB2 knockout MCF7 cell lysate (AB275010)

Knockout achieved by CRISPR/Cas9; X = 1 bp insertion; Frameshift : 100%