Human SCD5 knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human SCD5 knockout HeLa cell lysate (AB258650)
Allele-2 : Insertion of the selection cassette in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human SCD5 knockout HeLa cell lysate (AB258650)
Allele-3 : Insertion of the selection cassette in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human SCD5 knockout HeLa cell lysate (AB258650)
Allele-1 : 8 bp deletion in exon2
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Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The conversion of saturated fatty acids to monounsaturated fatty acids by SCD5 plays a significant role in maintaining lipid homeostasis. SCD5 does not form part of a larger protein complex refraining from complex interactions unlike some other SCD family members. The enzyme regulates the fluidity of cell membranes and is involved in synthesizing triglycerides and phospholipids affecting cellular energy storage and membrane composition.
Pathways
SCD5 is an important component in the lipid biosynthetic pathway and fatty acid metabolism. It functions alongside other enzymes in fatty acid desaturation pathways working closely with substrates produced by Acetyl-CoA Carboxylase (ACC) and Fatty Acid Synthase (FAS). These enzymes contribute to the synthesis and modification of lipids integrating into broader lipid signaling and metabolic pathways. SCD5's activity influences signaling pathways related to metabolism and energy balance within cells.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com