JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB263339

Human SCFD2 knockout HeLa cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

SCFD2 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.
1 Images
Sanger Sequencing - Human SCFD2 knockout HeLa cell lysate (AB263339)
  • Sanger seq

Unknown

Sanger Sequencing - Human SCFD2 knockout HeLa cell lysate (AB263339)

Homozygous : Insertion of the selection cassette in exon 1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.

Disease

Adenocarcinoma

style
left-column

Complementary Products

Cell Lines & Lysates

AB264851

Human SCFD2 knockout HeLa cell line

Sanger Sequencing - Human SCFD2 knockout HeLa cell line (AB264851)

  • 0
  • 0
style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab263339-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human SCFD2 knockout HeLa cell lysate", "number":"AB263339-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB263339-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
SCFD2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCFD2 also known as Sec1 family domain-containing protein 2 is a protein with a mass of approximately 42 kDa. It functions as an essential component in vesicle-mediated transport processes. SCFD2 is highly expressed in the brain testis and liver which indicates its potential relevance in multiple organ systems. Mechanically SCFD2 plays an active role in vesicle docking and fusion events mainly by interacting with SNARE complexes facilitating the release of vesicle contents at the target membrane.
Biological function summary

The function of SCFD2 is closely linked with the regulation of neurotransmitter release and protein trafficking within cells. Though it does not act alone SCFD2 associates with the exocyst complex involving several additional proteins critical for the proper localization and functioning of vesicles. This coordination ensures efficient cellular communication and contributes to normal cellular processes such as synaptic transmission and intracellular signaling.

Pathways

The role of SCFD2 extends into the vesicular transport and exocytosis pathways. It assists in the functioning of the SNARE machinery important for these pathways by interacting with proteins like SNAP-25 and syntaxin. These interactions highlight SCFD2's contribution to the dynamics of vesicle movement and release which are fundamental aspects of cellular homeostasis and responsiveness.

Disruptions or mutations in SCFD2 have connections with neurological conditions and neurodegenerative diseases including Alzheimer's disease. Its association with Alzheimer's is partly mediated through its relationship with other key proteins involved in the disease's pathways such as amyloid precursor protein (APP) and tau. Research into SCFD2 continues to clarify its precise roles in various diseases suggesting its potential as a therapeutic target.
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com