Human SCNN1D knockout HeLa cell lysate
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SCNN1D KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon8 and 2 bp deletion in exon8.
View Alternative Names
Amiloride-sensitive sodium channel subunit delta, DNACH, Delta-ENaC, Delta-NaCH, ENaC Delta, ENaCD, Epithelial Na(+) channel subunit delta, MGC149710, MGC149711, Nonvoltage-gated sodium channel 1 subunit delta, SCNED, SCNND_HUMAN, Sodium channel, nonvoltage gated 1, delta
- Sanger seq
Unknown
Sanger Sequencing - Human SCNN1D knockout HeLa cell lysate (AB259098)
Allele-2 : 1 bp deletion in exon8
- Sanger seq
Unknown
Sanger Sequencing - Human SCNN1D knockout HeLa cell lysate (AB259098)
Allele-1 : 2 bp deletion in exon8
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SCNN1D plays an important role in sodium reabsorption as part of the epithelial sodium channel complex which includes alpha beta and gamma subunits. Together these subunits form a channel responsible for the movement of sodium ions into the cells critical for maintaining electrolyte balance and blood pressure. Its specific contribution enhances the channel's sensitivity and responsiveness to hormonal regulation particularly by aldosterone which modulates electrolyte transport further influencing fluid accumulations in tissues.
Pathways
SCNN1D acts within the aldosterone-regulated sodium reabsorption pathway and the renin-angiotensin-aldosterone system (RAAS). These pathways are essential in regulating blood pressure and fluid balance. SCNN1D works closely with other proteins like the mineralocorticoid receptor which influences ENaC activity by regulating its transcription under the stimulus of aldosterone ensuring facilitating rapid sodium uptake in epithelial cells.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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