SCRIB KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon4 and Insertion of the selection cassette in exon4.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon4 and Insertion of the selection cassette in exon4.
Alternative names
CRIB 1, LAP 4, PDZ domain protein scribble, Protein LAP4, Protein scribble homolog, SCRIB 1, SCRIB_HUMAN, Scribble homolog 1, Vartul, hScrib, scribbled homolog (Drosophila)
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SCRIB KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon4 and Insertion of the selection cassette in exon4.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon4 and Insertion of the selection cassette in exon4.
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- SCRIB
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing, Western blot
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SCRIBBLE also known as SCRIB or Scribble homolog is a protein that mainly functions in cell polarity and adhesion. It has a mass of about 215 kDa. SCRIBBLE interacts with other cellular structures to maintain apical-basal polarity. Researchers observe its expression in several tissue types especially in epithelial tissues where maintaining cell polarity is important. SCRIBBLE acts as a scaffold that links signaling molecules and components of membranes facilitating the organization of molecular assemblies on the plasma membrane.
- Biological function summary
SCRIBBLE contributes significantly to cell orientation and tissue architecture. It is a critical component of the Scribble complex which also includes the proteins LGL1 and DLG1. This complex modulates cell junctions and the establishment of cell polarity. SCRIBBLE plays roles in processes such as wound healing and neural growth by modulating cell migration. It also influences epithelial-mesenchymal transition (EMT) a process important in development and cancer metastasis.
- Pathways
SCRIBBLE is integral in the regulation of the Hippo signaling pathway. Proper function of this pathway is necessary for controlling organ size and suppressing tumorigenesis. SCRIBBLE interacts with proteins such as YAP and TAZ which act as transcriptional co-activators within the Hippo pathway. Additionally SCRIBBLE is linked to the Wnt signaling pathway where it interacts with Disheveled proteins impacting processes such as cell fate and proliferation.
- Associated diseases and disorders
SCRIBBLE is implicated in cancer progression particularly in breast and colorectal cancers. Disruption in SCRIBBLE's function or expression can lead to loss of cell polarity and increased invasiveness contributing to tumor development. In breast cancer its interaction with the protein LATS2 in the Hippo pathway is important for controlling cell proliferation and apoptosis. Abnormal SCRIBBLE expression is also associated with neural tube defects where proper cell polarity and signaling are required for normal neural development.
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3 product images
Western blot - Human SCRIB (SCRIBBLE) knockout HeLa cell lysate (ab257660)
Lane 1: Wild-type HeLa cell lysate (20 ug)
Lane 2: SCRIB knockout HeLa cell lysate (20 ug)Lanes 1-2: Merged signal (red and green). Green - Anti-SCRIBBLE antibody [EPR4140(2)] ab125080 observed at 240 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
Anti-SCRIBBLE antibody [EPR4140(2)] ab125080 Anti-SCRIBBLE antibody [EPR4140(2)] was shown to specifically react with SCRIBBLE in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human SCRIB (SCRIBBLE) knockout HeLa cell line ab265190 (knockout cell lysate ab257660) was used. Wild-type and SCRIBBLE knockout samples were subjected to SDS-PAGE. Anti-SCRIBBLE antibody [EPR4140(2)] ab125080 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (Anti-SCRIBBLE antibody [EPR4140(2)] ab125080) at 1/500 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: SCRIB knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human SCRIB (SCRIBBLE) knockout HeLa cell line (Human SCRIB (SCRIBBLE) knockout HeLa cell line ab265190)
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 175 kDa
Observed band size: 240 kDa
Sanger Sequencing - Human SCRIB (SCRIBBLE) knockout HeLa cell lysate (ab257660)
Allele-1: 7 bp deletion in exon4
Sanger Sequencing - Human SCRIB (SCRIBBLE) knockout HeLa cell lysate (ab257660)
Allele-2: Insertion of the selection cassette in exon4
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