Human SCYL1 knockout HEK-293T cell lysate
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SCYL1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2.
View Alternative Names
CVAK90, Coated vesicle-associated kinase of 90 kDa, GKLP, HT019, Likely ortholog of mouse N terminal kinase like protein, MGC78454, N terminal kinase like, N-terminal kinase-like protein, NKTL, NTKL, NTKLBP1, NTKL_HUMAN, P105, SCY1 like 1, SCY1 like 1 protein 1, SCY1-like protein 1, SCYL1BP1, TAPK, TEIF, TR AP, Telomerase regulation-associated protein, Telomerase transcriptional element-interacting factor, Teratoma-associated tyrosine kinase
- Sanger seq
Unknown
Sanger Sequencing - Human SCYL1 knockout HEK-293T cell lysate (AB259099)
Homozygous : Insertion of the selection cassette in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
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Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SCYL1 contributes to the sorting and transport of proteins between the Golgi apparatus and the endoplasmic reticulum. It interacts with coatomer complexes to facilitate vesicular trafficking ensuring proper cellular function and protein homeostasis. As part of the cellular transport machinery SCYL1 helps maintain the structural integrity of the Golgi complex and likely plays role in neurodevelopment due to its expression patterns and involvement in protein handling.
Pathways
SCYL1 influences the Golgi-to-ER transport pathways and is involved in vesicle formation and movement. It works alongside proteins like coatomer protein complex subunits to maintain efficient vesicular transport within the cell. Through these pathways SCYL1 connects to broader cellular functions like protein sorting and endosome-to-Golgi retrieval contributing to the systematic recycling and allocation of proteins across the cellular framework.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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