Human SDF2L1 knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human SDF2L1 knockout HEK-293T cell lysate (AB258652)
Homozygous : 16 bp deletion in exon 1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SDF2L1 assists in maintaining ER function during cellular stress conditions. While not part of a large well-defined complex it interacts with multiple proteins to support ER stress responses and the unfolded protein response (UPR). This activity helps protect cells from dysfunction associated with accumulation of misfolded proteins and ensures the degradation or refolding of faulty proteins. As a result SDF2L1 plays a role in cellular adaptation to stress especially during periods of increased protein load.
Pathways
SDF2L1 acts in several interconnected processes involving ER stress and the UPR pathway. It interacts with proteins such as BiP/GRP78 and calnexin which are key components of the UPR. These interactions help modulate the load of unfolded proteins within the ER ensuring that cell survival mechanisms are activated appropriately. SDF2L1’s role in these pathways highlights its importance in regulating cellular stress responses and maintaining cellular equilibrium.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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