Human SDF4 knockout HEK-293T cell lysate
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SDF4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 11 bp deletion in exon 2.
View Alternative Names
45 kDa calcium-binding protein, CAB45_HUMAN, Calcium binding protein, PSEC0034, Stromal cell-derived factor 4
- Sanger seq
Unknown
Sanger Sequencing - Human SDF4 knockout HEK-293T cell lysate (AB258653)
Homozygous : 11 bp deletion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein participates in the regulation of calcium-dependent vesicle transport. It does not function as part of a large protein complex but interacts with different proteins in the Golgi for sorting activities. SDF4's ability to bind calcium allows it to modulate interactions necessary for vesicle movement and cargo recognition affecting how proteins exit the Golgi and are distributed to other areas of the cell.
Pathways
Studies show SDF4 is involved in the cellular secretory pathway and calcium signaling pathway. Within these pathways SDF4 interacts with proteins like co-chaperone SIL1 to ensure proper protein folding and trafficking. SDF4 also contributes to vesicle budding processes that are essential for maintaining proper cellular function and homeostasis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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