Human SDHAF2 (C11orf79) knockout HeLa cell lysate
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SDHAF2 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 2 bp deletion in exon1.
View Alternative Names
Chromosome 11 open reading frame 79, FLJ20487, PGL2, Paraganglioma or familial glomus tumors 2, SDH assembly factor 2, SDH5, SDHF2_HUMAN, Sdhaf2, Succinate dehydrogenase assembly factor 2, mitochondrial, Succinate dehydrogenase subunit 5, hSDH5, mitochondrial
- Sanger seq
Unknown
Sanger Sequencing - Human SDHAF2 (C11orf79) knockout HeLa cell lysate (AB263341)
Allele-1 : 2 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human SDHAF2 (C11orf79) knockout HeLa cell lysate (AB263341)
Allele-2 : 1 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
C11orf79 impacts cellular processes in the immune system and has links to the HAPSTR complex where it interacts with other proteins to regulate hematopoiesis. The protein contributes to maintaining normal cell function by aiding in the regulation of proliferation and differentiation of myeloid and lymphoid cells. This suggests its involvement in general immune response and hematopoietic homeostasis.
Pathways
C11orf79 participates in pathways related to immune cell development and function. Notably it engages in the Notch signaling pathway which influences hematopoietic cell differentiation and lymphocyte development. Additionally it interacts with proteins such as NOTCH1 and RBPJ which are essential in both the regulation of developmental processes and maintaining cellular identity.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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