Human SEC11A (SPC18) knockout HEK-293T cell lysate
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SEC11A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 31 bp deletion in exon 2.
View Alternative Names
1810012E07Rik, Endopeptidase SP18, Microsomal signal peptidase 18 kDa subunit, SC11A_HUMAN, SEC11 homolog A, SEC11 homolog A (S. cerevisiae), SEC11-like protein 1, SEC11A, SEC11L1, SPC18, SPCS4A, SPase 18 kDa subunit, Signal peptidase complex (18kD), Signal peptidase complex catalytic subunit SEC11A, sid2895
- Sanger seq
Unknown
Sanger Sequencing - Human SEC11A (SPC18) knockout HEK-293T cell lysate (AB258655)
Homozygous : 31 bp deletion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SPC18 functions as part of the yeast's spindle assembly complex. This complex plays an important role in regulating the organization and dynamics of spindle microtubules. SPC18 helps maintain spindle integrity and aids in anchoring microtubules to the spindle pole body. Its function is important for successful mitotic progression and ensures that chromosomes are accurately distributed into daughter cells.
Pathways
SPC18 is involved in the mitotic spindle assembly pathway. It interacts closely with proteins like Spc110 and Tub4 which are also part of the spindle pole body assembly. The mitotic pathway in which SPC18 plays a role ensures proper chromosome alignment and segregation during mitosis which is vital for genomic stability.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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