Human SEC13 (SEC13L1) knockout HEK-293T cell lysate
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SEC13 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon2.
View Alternative Names
D3S1231E, Npp 20, Protein SEC13 homolog, SEC13 (S. cerevisiae) like 1, SEC13 homolog, SEC13 homolog (S. cerevisiae), SEC13 like 1 (S. cerevisiae), SEC13 like 1 isoform, SEC13 protein, SEC13-like protein 1, SEC13-related protein, SEC13R, SEC13_HUMAN
- Sanger seq
Unknown
Sanger Sequencing - Human SEC13 (SEC13L1) knockout HEK-293T cell lysate (AB259101)
Homozygous : 2 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
As a part of the SEC13 complex SEC13L1 serves an essential function in the process of vesicular transport. This protein forms a core element of the COPII coat complex which is a multi-protein assembly responsible for the budding of transport vesicles from the endoplasmic reticulum. Besides its role in protein transport SEC13L1 also participates in maintaining the architecture of the nuclear pore complex which is critical for nucleocytoplasmic transport.
Pathways
SEC13L1 plays an important role in the secretory pathway and is involved in the ER-Golgi transport pathway. It associates closely with proteins like SEC24 and SEC23 which work together in the COPII coat formation. The interaction with these proteins is important for the selection of cargo proteins that are exported in COPII vesicles. Furthermore SEC13L1 is also involved in the regulation of nucleocytoplasmic transport pathways alongside proteins such as NUP107 as part of the nuclear pore complex degradation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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