Human SEC61G knockout HEK-293T cell lysate
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SEC61G KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 2 bp deletion in exon 2.
View Alternative Names
Protein transport protein Sec61 subunit gamma, SC61G_HUMAN, SEC61G, SSS1, Sec61 gamma subunit
- Sanger seq
Unknown
Sanger Sequencing - Human SEC61G knockout HEK-293T cell lysate (AB259102)
Allele-1 : 2 bp deletion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human SEC61G knockout HEK-293T cell lysate (AB259102)
Allele-2 : 1 bp deletion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The SEC61G protein operates as part of the Sec61 complex which includes Sec61 alpha and beta subunits. This complex is essential for protein translocation in the ER. The complex not only facilitates the entry of nascent polypeptides into the ER but also aids in the integration of membrane proteins. This process is important for protein turnover and cellular homeostasis.
Pathways
SEC61G takes part in the protein processing in the endoplasmic reticulum and the unfolded protein response pathways. The Sec61 complex works in conjunction with the signal recognition particle and docking protein to efficiently translocate proteins. Moreover SEC61G interacts with proteins like BiP and calnexin during protein folding and quality control within the ER.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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