Human SECTM1 knockout A549 cell lysate
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SECTM1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon2 and 1 bp insertion in exon2.
View Alternative Names
K12, K12 protein, Protein K-12, SCTM1_HUMAN, Secreted and transmembrane 1, Secreted and transmembrane protein 1, Type 1a transmembrane protein
- Sanger seq
Unknown
Sanger Sequencing - Human SECTM1 knockout A549 cell lysate (AB259103)
Allele-2 : 1 bp insertion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human SECTM1 knockout A549 cell lysate (AB259103)
Allele-1 : 17 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein plays a role in modulating immune responses. SECTM1 interacts with CD7 a cell surface glycoprotein found on T cells and natural killer cells influencing their activation and proliferation. This interaction is important for immune system regulation and SECTM1 can function together with other immune-related proteins but usually operates independently rather than as part of a larger protein complex.
Pathways
SECTM1 is involved in immunological pathways particularly those governing T-cell activation and the broader inflammatory response. Within these pathways SECTM1 connects with proteins like CD7 and is associated with the PI3K/Akt signaling cascade. This linkage influences cell survival and growth bridging innate and adaptive immunity.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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