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SELENBP1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 2 and 1 bp insertion in exon 2.

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Images

Western blot - Human SELENBP1 (Selenium Binding Protein 1/SBP) knockout HeLa cell lysate (AB257662), expandable thumbnail
  • Sanger Sequencing - Human SELENBP1 (Selenium Binding Protein 1/SBP) knockout HeLa cell lysate (AB257662), expandable thumbnail
  • Sanger Sequencing - Human SELENBP1 (Selenium Binding Protein 1/SBP) knockout HeLa cell lysate (AB257662), expandable thumbnail

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing, Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 2 and 1 bp insertion in exon 2.

Alternative names

What's included?

1 Kit
Components
Human SELENBP1 knockout HeLa cell lysate
1 x 100 µg
Human wild-type HeLa cell lysate
1 x 100 µg

Recommended products

SELENBP1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 2 and 1 bp insertion in exon 2.

Key facts

Cell type

HeLa

Mutation description

Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 2 and 1 bp insertion in exon 2.

Disease

Adenocarcinoma

Concentration
Loading...

Properties

Gene name

SELENBP1

Gene editing type

Knockout

Gene editing method

CRISPR technology

Knockout validation

Sanger Sequencing, Western blot

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Notes


Knockout cell lysate achieved by CRISPR/Cas9.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Selenium Binding Protein 1 (SBP1) also known as SELENBP1 is a protein of molecular weight approximately 56 kDa. It is involved in various cellular mechanisms. SBP1 binds selenium thereby playing a role in selenium metabolism and regulation within the cell. It is expressed in numerous tissues including liver kidney and colon indicating a broad physiological significance. Its expression is subject to tight regulation pointing to its importance in cellular functions.

Biological function summary

Selenium Binding Protein 1 interacts with selenium implicating it in various metabolic processes. It does not act as part of a larger complex but functions independently to influence cellular responses to selenium levels. Research shows that SBP1 plays a role in antioxidant defense and cellular detoxification suggesting a protective function against oxidative stress. This regulation supports various metabolic pathways by modulating selenium-dependent enzyme activity.

Pathways

Selenium Binding Protein 1 has been connected to the antioxidant defense pathway and the detoxification process. It interacts with proteins involved in these pathways such as glutathione peroxidases essential for eliminating oxidative damage in cells. By influencing these pathways SBP1 helps maintain cellular homeostasis highlighting its role in the broader metabolic network.

Associated diseases and disorders

Aberrations in Selenium Binding Protein 1 levels are linked to certain cancers and neurological disorders. Studies show a decrease in SBP1 expression in cancers such as colorectal cancer possibly related to reduced cellular detoxification capacity. Additionally some neurological disorders have shown altered SBP1 expression which could connect the protein with enhanced oxidative stress and cell damage. By affecting conditions like cancer SBP1 could interact with proteins involved in cell proliferation and survival supporting research on therapeutic targets.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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