Human SELENBP1 (Selenium Binding Protein 1/SBP) knockout HeLa cell lysate
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(0 Publication)
- WB
Unknown
Western blot - Human SELENBP1 (Selenium Binding Protein 1/SBP) knockout HeLa cell lysate (AB257662)
Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : SELENBP1 knockout HeLa cell lysate (20 ug)
Lane 3 : HT-29 cell lysate (20 ug)
Lane 4 : SW 480 cell lysate (20 ug)
Lanes 1-3 : Merged signal (red and green). Green - ab90135 observed at 60 kDa. Red - loading control ab8245 observed at 36 kDa.
ab90135 Anti-Selenium Binding Protein 1/SBP antibody was shown to specifically react with Selenium Binding Protein 1/SBP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265279 (knockout cell lysate ab257662) was used. Wild-type and Selenium Binding Protein 1/SBP knockout samples were subjected to SDS-PAGE. ab90135 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Selenium Binding Protein 1/SBP antibody (<a href='/en-us/products/primary-antibodies/selenium-binding-protein-1-sbp-antibody-ab90135'>ab90135</a>) at 1/500 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
SELENBP1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human SELENBP1 (Selenium Binding Protein 1/SBP) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-selenbp1-selenium-binding-protein-1-sbp-knockout-hela-cell-line-ab265279'>ab265279</a>)
Lane 3:
HT-29 cell lysate at 20 µg
Lane 4:
SW 480 cell lysate at 20 µg
Secondary
Lanes 1 - 4:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Lane 4:
<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a> at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 60 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human SELENBP1 (Selenium Binding Protein 1/SBP) knockout HeLa cell lysate (AB257662)
Allele-2 : 1 bp insertion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human SELENBP1 (Selenium Binding Protein 1/SBP) knockout HeLa cell lysate (AB257662)
Allele-1 : 11 bp deletion in exon 2
Complementary Products
Cell Lines & Lysates
AB265279
Human SELENBP1 (Selenium Binding Protein 1/SBP) knockout HeLa cell line
Advanced Validation
- 0
- 0
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Selenium Binding Protein 1 interacts with selenium implicating it in various metabolic processes. It does not act as part of a larger complex but functions independently to influence cellular responses to selenium levels. Research shows that SBP1 plays a role in antioxidant defense and cellular detoxification suggesting a protective function against oxidative stress. This regulation supports various metabolic pathways by modulating selenium-dependent enzyme activity.
Pathways
Selenium Binding Protein 1 has been connected to the antioxidant defense pathway and the detoxification process. It interacts with proteins involved in these pathways such as glutathione peroxidases essential for eliminating oxidative damage in cells. By influencing these pathways SBP1 helps maintain cellular homeostasis highlighting its role in the broader metabolic network.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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