SENP5 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon2 and Insertion of the selection cassette in exon2.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon2 and Insertion of the selection cassette in exon2.
Alternative names
FKSG45, FLJ42398, MGC27076, Protease FKSG45, SENP5_HUMAN, SUMO specific protease SENP5, SUMO1/sentrin specific peptidase 5, SUMO1/sentrin specific protease 5, Sentrin specific protease SENP5, Sentrin-specific protease 5, Sentrin/SUMO-specific protease SENP5
What's included?
Recommended products
SENP5 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon2 and Insertion of the selection cassette in exon2.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon2 and Insertion of the selection cassette in exon2.
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- SENP5
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SENP5 also known as SUMO-specific protease 5 is an enzyme involved in the processing and deconjugation of SUMO proteins. It has an approximate mass of 82 kDa. SENP5 is expressed mainly in the heart skeletal muscle and to a lesser extent in the testis. This enzyme functions by cleaving SUMO from target proteins enabling the regulation of protein function and localization. It plays a significant role in maintaining cellular homeostasis by reversing the small ubiquitin-like modifier (SUMO) modification cycle.
- Biological function summary
SENP5 removes SUMO modifications from proteins involved in cellular processes such as mitotic progression and mitochondrial dynamics. It is not part of a complex but interacts with various cellular proteins to facilitate its role in SUMOylation regulation. By controlling the SUMOylation status SENP5 influences cell cycle progression and cellular stress responses. SENP5 also contributes to mitochondrial morphology by modifying proteins related to mitochondrial fission and fusion processes.
- Pathways
SENP5 interacts within the SUMOylation pathway and the mitochondrial pathway. In the SUMOylation pathway SENP5 works alongside other SENP family proteins such as SENP1 and SENP2 which share similar functional roles in SUMO processing. In the mitochondrial pathway SENP5 is linked to the regulation of proteins like DRP1 which is essential for mitochondrial fission. The interactions in these pathways demonstrate SENP5's significant role in cellular homeostasis and energy metabolism.
- Associated diseases and disorders
SENP5's dysregulation has been associated with cardiomyopathy and certain types of cancer. In cardiomyopathy altered SENP5 activity can lead to disrupted mitochondrial dynamics and impaired cardiac function. Its interaction with mitochondrial proteins such as DRP1 illustrates its importance in cardiac cell health. In cancer SENP5's role in cell cycle regulation is important as alterations can lead to uncontrolled cell proliferation. SENP5's interaction with proteins involved in the cell cycle highlights its potential impact on tumor growth and development.
Product promise
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2 product images
Sanger Sequencing - Human SENP5 knockout HeLa cell lysate (ab259105)
Allele-2: Insertion of the selection cassette in exon2
Sanger Sequencing - Human SENP5 knockout HeLa cell lysate (ab259105)
Allele-1: 2 bp insertion in exon2
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