Human SENP5 knockout HeLa cell lysate
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SENP5 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon2 and Insertion of the selection cassette in exon2.
View Alternative Names
FKSG45, FLJ42398, MGC27076, Protease FKSG45, SENP5_HUMAN, SUMO specific protease SENP5, SUMO1/sentrin specific peptidase 5, SUMO1/sentrin specific protease 5, Sentrin specific protease SENP5, Sentrin-specific protease 5, Sentrin/SUMO-specific protease SENP5
- Sanger seq
Unknown
Sanger Sequencing - Human SENP5 knockout HeLa cell lysate (AB259105)
Allele-1 : 2 bp insertion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human SENP5 knockout HeLa cell lysate (AB259105)
Allele-2 : Insertion of the selection cassette in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SENP5 removes SUMO modifications from proteins involved in cellular processes such as mitotic progression and mitochondrial dynamics. It is not part of a complex but interacts with various cellular proteins to facilitate its role in SUMOylation regulation. By controlling the SUMOylation status SENP5 influences cell cycle progression and cellular stress responses. SENP5 also contributes to mitochondrial morphology by modifying proteins related to mitochondrial fission and fusion processes.
Pathways
SENP5 interacts within the SUMOylation pathway and the mitochondrial pathway. In the SUMOylation pathway SENP5 works alongside other SENP family proteins such as SENP1 and SENP2 which share similar functional roles in SUMO processing. In the mitochondrial pathway SENP5 is linked to the regulation of proteins like DRP1 which is essential for mitochondrial fission. The interactions in these pathways demonstrate SENP5's significant role in cellular homeostasis and energy metabolism.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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