Human SEPHS1 (Selenophosphate synthetase 1) knockout HEK-293T cell lysate
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SEPHS1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
View Alternative Names
MGC4980, SELD, SEPHS1, SPS, SPS1_HUMAN, Selenide, Selenide, water dikinase 1, Selenium donor protein 1, Selenophosphate synthase 1, Selenophosphate synthetase 1, water dikinase 1
- Sanger seq
Unknown
Sanger Sequencing - Human SEPHS1 (Selenophosphate synthetase 1) knockout HEK-293T cell lysate (AB263342)
Homozygous : 1 bp insertion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein is involved in the synthesis of selenoproteins important for cellular antioxidant defense and redox homeostasis. Selenophosphate synthetase 1 functions as part of a multi-enzyme complex essential for the biosynthesis of selenocysteine the 21st amino acid which is then incorporated into selenoproteins. These selenoproteins include glutathione peroxidases and thioredoxin reductases which are important for maintaining cellular integrity.
Pathways
The protein participates actively in the selenocysteine biosynthesis and recycling pathway ensuring adequate supply of selenophosphate needed for selenoprotein production. Within this pathway SEPHS1 closely associates with several enzymes like selenocysteine synthase and selenocysteine tRNA (tRNASec) which facilitate the incorporation of selenocysteine into proteins. These interactions highlight the interconnected role of SEPHS1 in maintaining proper cellular functions through selenoproteins.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Cell Lines & Lysates
AB266115
Human SEPHS1 (Selenophosphate synthetase 1) knockout HEK-293T cell line
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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