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SERBP1 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 23 bp deletion in exon 1 and 29 bp deletion in exon 1.

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Images

Sanger Sequencing - Human SERBP1 knockout HEK-293T cell lysate (AB258658), expandable thumbnail
  • Sanger Sequencing - Human SERBP1 knockout HEK-293T cell lysate (AB258658), expandable thumbnail

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 23 bp deletion in exon 1 and 29 bp deletion in exon 1.

Alternative names

What's included?

1 Kit
Components
Human SERBP1 knockout HEK293T cell lysate
1 x 100 µg
Human wild-type HEK293T cell lysate
1 x 100 µg

Recommended products

SERBP1 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 23 bp deletion in exon 1 and 29 bp deletion in exon 1.

Key facts

Cell type

HEK-293T

Mutation description

Knockout achieved by using CRISPR/Cas9, 23 bp deletion in exon 1 and 29 bp deletion in exon 1.

Concentration
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Properties

Gene name

SERBP1

Gene editing type

Knockout

Gene editing method

CRISPR technology

Knockout validation

Sanger Sequencing

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Notes


Knockout cell lysate achieved by CRISPR/Cas9.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SERBP1 also known as SCR3 is a protein weighing approximately 46 kDa. This protein interacts with specific mRNA sequences acting as a binding protein that influences mRNA stability and modulates the translation process. SERBP1 is widely expressed in various tissues notably in the liver and placenta where it plays a role in cellular activities related to protein synthesis.

Biological function summary

Activities performed by SERBP1 involve regulation of mRNA turnover and translation impacting critical cellular functions. SERBP1 forms part of the ribonucleoprotein complexes which bind to mRNA and stabilize it therefore influencing gene expression. Through these interactions it has a significant impact on the control of translation processes within the cell alongside other regulatory proteins.

Pathways

SERBP1 participates in mRNA surveillance and regulation pathways. This protein interacts with key translation pathways contributing to processes that ensure only properly processed mRNA is translated into proteins. SERBP1's role in these pathways may involve interactions with proteins like eIF4E which also plays a part in translation initiation thereby maintaining cellular homeostasis and efficiency in protein production.

Associated diseases and disorders

Changes in SERBP1 expression or function can relate to cancer and liver disease. In such conditions aberrant regulation of mRNA stability may occur leading to dysregulated protein synthesis that contributes to pathogenesis. SERBP1 is connected to proteins such as c-Myc in cancer where it may play a role in the overexpression of oncogenes linking these pathway interactions to disease states.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sanger Sequencing - Human SERBP1 knockout HEK-293T cell lysate (ab258658), expandable thumbnail

    Sanger Sequencing - Human SERBP1 knockout HEK-293T cell lysate (ab258658)

    Allele-1: 29 bp deletion in exon 1

  • Sanger Sequencing - Human SERBP1 knockout HEK-293T cell lysate (ab258658), expandable thumbnail

    Sanger Sequencing - Human SERBP1 knockout HEK-293T cell lysate (ab258658)

    Allele-2: 23 bp deletion in exon 1

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com